This 6 ounce bottle will treat a 5,000 gallon pond for 6 months. Or a treat a 2,500 gallon pond for 13 months. Microbe-lift biological mosquito control can be applied to areas that can contain aquatic life, fish and plants. This mosquito control product can also be applied to areas used by or in contact with humans, animals, horses, livestock, pets birds or wildlife. Biological larvacide, bacillus thuringiensis subspecies israaelenses (B.T.I.) Is the active ingredient). Can be used in water gardens that contain live fish and plants. Up to 14 days activity depending on application site. Disperses evenly in water. No fish toxicity. No toxicity to non-target invertebrates. No potential for resistance development in the target mosquito population.